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rna stat60 kit  (AMS Biotechnology)


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    Structured Review

    AMS Biotechnology rna stat60 kit
    Rna Stat60 Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+stat60+kit/RNA+STAT-60/med_rxiv__2025__05__27__25328314-195-15-18
    Average 96 stars, based on 559 article reviews
    rna stat60 kit - by Bioz Stars, 2026-09
    96/100 stars

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    Polymerase Chain Reaction:

    Article Title: Role of histone modification in 12‑lipoxygenase‑associated p21 gene regulation.
    Article Snippet: .. An RNA STAT60 kit (AMS Biotechnology Europe Ltd., Abingdon, UK) was used to extract the total mRNA from the MCs, and a TaqMan PreAmp Master mix (Applied Biosystems; Thermo Fisher Scientific, Inc.) was then used to reverse transcribe 1.5 μg mRNA into cDNA, the final product was diluted 10‐fold and used as the template for PCR amplification. .. An ABI‐7500 real‐time quantitative PCR instrument (Thermo Fisher Scientific, Inc.) was used, together with the SYBR Green PCR Master Mix kit (Thermo Fisher Scientific, Inc.).

    Amplification:

    Article Title: Role of histone modification in 12‑lipoxygenase‑associated p21 gene regulation.
    Article Snippet: .. An RNA STAT60 kit (AMS Biotechnology Europe Ltd., Abingdon, UK) was used to extract the total mRNA from the MCs, and a TaqMan PreAmp Master mix (Applied Biosystems; Thermo Fisher Scientific, Inc.) was then used to reverse transcribe 1.5 μg mRNA into cDNA, the final product was diluted 10‐fold and used as the template for PCR amplification. .. An ABI‐7500 real‐time quantitative PCR instrument (Thermo Fisher Scientific, Inc.) was used, together with the SYBR Green PCR Master Mix kit (Thermo Fisher Scientific, Inc.).

    Isolation:

    Article Title: PTH Hypersecretion Triggered by a GABA B1 and Ca 2+ -sensing Receptor Heterocomplex in Hyperparathyroidism
    Article Snippet: Serum samples were prepared by centrifugation and analyzed for total serum calcium by an automated ACE Alera Clinical Chemistry bioanalyzer (Alfa Wassermann, Inc, West Caldwell, NJ, USA) and serum PTH by commercial intact PTH ELISA kit (Immutopics, San Clemente, Ca, USA), following manufacturer instructions. .. Total RNA was isolated from human adenomas and microdissected mouse PTGs devoid of thyroid and fibrous tissues by an RNA STAT60 kit (Amsbio LLC, Cambridge, MA), reverse transcribed by M-MLV reverse transcriptase into cDNA, and subjected to qPCR for gene expression profiles using commercial TaqMan-based sets of primers and probes (Applied Biosystems). ..

    Article Title: β-Amyloid as a new target to suppress tonic PTH hypersecretion in primary hyperparathyroidism
    Article Snippet: .. Total RNA was isolated from human parathyroid adenomas and PTGs of normal donors by an RNA STAT60 kit (Amsbio LLC, Cambridge, MA). ..

    Reverse Transcription:

    Article Title: PTH Hypersecretion Triggered by a GABA B1 and Ca 2+ -sensing Receptor Heterocomplex in Hyperparathyroidism
    Article Snippet: Serum samples were prepared by centrifugation and analyzed for total serum calcium by an automated ACE Alera Clinical Chemistry bioanalyzer (Alfa Wassermann, Inc, West Caldwell, NJ, USA) and serum PTH by commercial intact PTH ELISA kit (Immutopics, San Clemente, Ca, USA), following manufacturer instructions. .. Total RNA was isolated from human adenomas and microdissected mouse PTGs devoid of thyroid and fibrous tissues by an RNA STAT60 kit (Amsbio LLC, Cambridge, MA), reverse transcribed by M-MLV reverse transcriptase into cDNA, and subjected to qPCR for gene expression profiles using commercial TaqMan-based sets of primers and probes (Applied Biosystems). ..

    Real-time Polymerase Chain Reaction:

    Article Title: PTH Hypersecretion Triggered by a GABA B1 and Ca 2+ -sensing Receptor Heterocomplex in Hyperparathyroidism
    Article Snippet: Serum samples were prepared by centrifugation and analyzed for total serum calcium by an automated ACE Alera Clinical Chemistry bioanalyzer (Alfa Wassermann, Inc, West Caldwell, NJ, USA) and serum PTH by commercial intact PTH ELISA kit (Immutopics, San Clemente, Ca, USA), following manufacturer instructions. .. Total RNA was isolated from human adenomas and microdissected mouse PTGs devoid of thyroid and fibrous tissues by an RNA STAT60 kit (Amsbio LLC, Cambridge, MA), reverse transcribed by M-MLV reverse transcriptase into cDNA, and subjected to qPCR for gene expression profiles using commercial TaqMan-based sets of primers and probes (Applied Biosystems). ..

    Gene Expression:

    Article Title: PTH Hypersecretion Triggered by a GABA B1 and Ca 2+ -sensing Receptor Heterocomplex in Hyperparathyroidism
    Article Snippet: Serum samples were prepared by centrifugation and analyzed for total serum calcium by an automated ACE Alera Clinical Chemistry bioanalyzer (Alfa Wassermann, Inc, West Caldwell, NJ, USA) and serum PTH by commercial intact PTH ELISA kit (Immutopics, San Clemente, Ca, USA), following manufacturer instructions. .. Total RNA was isolated from human adenomas and microdissected mouse PTGs devoid of thyroid and fibrous tissues by an RNA STAT60 kit (Amsbio LLC, Cambridge, MA), reverse transcribed by M-MLV reverse transcriptase into cDNA, and subjected to qPCR for gene expression profiles using commercial TaqMan-based sets of primers and probes (Applied Biosystems). ..



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